CORE Equipment

Library Generation and Screening

 proflex pcr

PROFLEX PCR SYSTEM 3-in-1 THERMAL CYCLER

Applied Biosystems Thermo Fisher

  • Model 4484073
  • 2-Zone VeriFlex blocks
  • 3 blocks for 32 x 0.2 mL PCR tubes/block
 ibright fl

IBRIGHT FL1500 IMAGING SYSTEM

Invitrogen Thermo Fisher

  • DNA, SDS-PAGE, Western, and sample imaging
  • Five fluorescence channels
  • Smart Exposure technology
  • Automatic sample rotation, automatic zoom, and automatic focus
  • 22.5 x 18.0-cm field of view
  • Green LED-based transilluminator
  • Automatic colony counting
 bead ruptor

BEAD RUPTOR ELITE HOMOGENIZER

Omni International

  • Process 48 2 mL samples simultaneously
  • Improve RNA extractions
  • Improve yeast lysis

Protein Production and Purification

 two mutlitron

TWO MULTITRON CO2 INCUBATOR/SHAKERS

Infors HT

  • Shake speed, CO2, and humidity control
  • Up to 6 L of capacity for mammalian protein expression
 1300 series a2

1300 SERIES A2 CLASS II BIOSAFETY CABINET

Thermo Scientific

  • Sterile cabinet for mammalian cell culture
 celldrop

CELLDROP CELL COUNTER

DeNovix

  • Brightfield and fluorescent counting of mammalian cell suspensions

 avanti

avanti j 15r

AVANTI JE & AVANTI J-15R CENTRIFUGES

Beckman Coulter

  • Centrifugation of cultures and samples
  • Adapters for flow tubes, 15 mL and 50 mL conicals, Oakridge tubes, 500 mL bottles
 akta

AKTA PURE 25L FPLC

Cytiva

  • S75 and S200 size exclusion chromatography
  • Protein A, Protein G, Streptactin affinity columns
  • Ion exchange chromatography
  • Sample pump
  • Fraction collector

Protein Functional Characterization

 minidawn

MINIDAWN MULTI-ANGLE LIGHT SCATTERING

Wyatt Technology Waters

  • Downstream of AKTA Pure FPLC
  • Determination of absolute mass and size
  • Waters XBridge Premier Column – 10 to 650 kDa
 synergy h1

SYNERGY H1 MULTIMODE PLATE READER 

Agilent BioTek

  • Monochromator-based and filter-based optics
  • Automated Z-focus
  • 7 log measurement range
  • Take3 late for microvolume measurements
 attune

ATTUNE FLOW CYTOMETER 

Thermo Fisher

  • Violet (405 nm), blue (488 nm), yellow (562 nm), and red (638 nm) laser excitation
  • 14 fluorescence channels
  • CytKick for autosampling from 96-well plates
 cytation c10

CYTATION C10 CONFOCAL IMAGING READER 

Agilent BioTek

  • Automated laser confocal and widefield microscopy
  • Accommodates slides, dishes, microplates, and T25 flasks
  • Hamamatsu sCMOS camera
  • CO2 incubation with temperature control and shaking
  • 4×, 10×, 20×, 40×, 60× objectives
  • DAPI, GFP, RFP, CY5 confocal filter cubes
  • DAPI, GFP, Texas Red Widefield filter cubes

Longterm Sample Storage

 tsx ult

TSX ULT -80 ºC FREEZER  

Fisher Scientific

  • 600 box capacity
  • Minus80 monitoring
 vip plus

VIP PLUS SERIES -150 ºC CHEST FREEZER

BSI PHCbi

  • Storage of mammalian and primary cells and lines
  • 165 box capacity
  • Liquid nitrogen back up
  • Minus80 monitoring