Library Generation and Screening
PROFLEX PCR SYSTEM 3-in-1 THERMAL CYCLER
Applied Biosystems Thermo Fisher
- Model 4484073
- 2-Zone VeriFlex blocks
- 3 blocks for 32 x 0.2 mL PCR tubes/block
IBRIGHT FL1500 IMAGING SYSTEM
Invitrogen Thermo Fisher
- DNA, SDS-PAGE, Western, and sample imaging
- Five fluorescence channels
- Smart Exposure technology
- Automatic sample rotation, automatic zoom, and automatic focus
- 22.5 x 18.0-cm field of view
- Green LED-based transilluminator
- Automatic colony counting
BEAD RUPTOR ELITE HOMOGENIZER
Omni International
- Process 48 2 mL samples simultaneously
- Improve RNA extractions
- Improve yeast lysis
Protein Production and Purification
TWO MULTITRON CO2 INCUBATOR/SHAKERS
Infors HT
- Shake speed, CO2, and humidity control
-
Up to 6 L of capacity for mammalian protein expression
1300 SERIES A2 CLASS II BIOSAFETY CABINET
Thermo Scientific
- Sterile cabinet for mammalian cell culture
CELLDROP CELL COUNTER
DeNovix
- Brightfield and fluorescent counting of mammalian cell suspensions
AVANTI JE & AVANTI J-15R CENTRIFUGES
Beckman Coulter
- Centrifugation of cultures and samples
- Adapters for flow tubes, 15 mL and 50 mL conicals, Oakridge tubes, 500 mL bottles
AKTA PURE 25L FPLC
Cytiva
- S75 and S200 size exclusion chromatography
- Protein A, Protein G, Streptactin affinity columns
- Ion exchange chromatography
- Sample pump
- Fraction collector
Protein Functional Characterization
MINIDAWN MULTI-ANGLE LIGHT SCATTERING
Wyatt Technology Waters
- Downstream of AKTA Pure FPLC
- Determination of absolute mass and size
- Waters XBridge Premier Column – 10 to 650 kDa
SYNERGY H1 MULTIMODE PLATE READER
Agilent BioTek
- Monochromator-based and filter-based optics
- Automated Z-focus
- 7 log measurement range
- Take3 late for microvolume measurements
ATTUNE FLOW CYTOMETER
Thermo Fisher
- Violet (405 nm), blue (488 nm), yellow (562 nm), and red (638 nm) laser excitation
- 14 fluorescence channels
- CytKick for autosampling from 96-well plates
CYTATION C10 CONFOCAL IMAGING READER
Agilent BioTek
- Automated laser confocal and widefield microscopy
- Accommodates slides, dishes, microplates, and T25 flasks
- Hamamatsu sCMOS camera
- CO2 incubation with temperature control and shaking
- 4×, 10×, 20×, 40×, 60× objectives
- DAPI, GFP, RFP, CY5 confocal filter cubes
- DAPI, GFP, Texas Red Widefield filter cubes
Longterm Sample Storage
TSX ULT -80 ºC FREEZER
Fisher Scientific
- 600 box capacity
- Minus80 monitoring
VIP PLUS SERIES -150 ºC CHEST FREEZER
BSI PHCbi
- Storage of mammalian and primary cells and lines
- 165 box capacity
- Liquid nitrogen back up
- Minus80 monitoring